SLU-PP-332

SLU-PP-332 | Pan-ERR Agonist for Metabolic and Mitochondrial Research

Chemical Name: SLU-PP-332
CAS No.: 303760-60-3
Molecular Formula: C₁₈H₁₄N₂O₂
Molecular Weight: 290.32 g/mol
Appearance: White to off-white solid
Purity: ≥98% (by HPLC)
Storage:

Powder: –20°C for up to 3 years

In Solution: –80°C (6 months); –20°C (1 month)
Shipping: Room temperature (domestic U.S.); conditions may vary internationally

Product Description
SLU-PP-332 is a potent, small-molecule pan-Estrogen-Related Receptor (ERR) agonist that exhibits strong affinity for ERR subtypes, with EC₅₀ values of:

ERRα: 98 nM

ERRβ: 230 nM

ERRγ: 430 nM

This compound is a valuable research tool for investigating mitochondrial biogenesis, aerobic metabolism, and skeletal muscle function, particularly in the context of metabolic diseases and obesity-related disorders.

Key Features
Target Specificity: Selective activation of ERRα, ERRβ, and ERRγ

Cellular Effectiveness: Promotes mitochondrial gene expression and respiration in C2C12 muscle cells

In Vivo Efficacy: Enhances oxidative muscle phenotype, induces fatty acid oxidation, and supports glucose metabolism in high-fat diet-induced obesity models

Versatile Applications: Ideal for studying metabolic health, muscle performance, and mitochondrial dysfunction

Biological Activity
In Vitro
Cell Line: C2C12 myocytes

Concentration: 0–5 μM

Incubation: 24 hours

Effect: Upregulates Pdk4, induces mitochondrial biogenesis, and enhances respiration

Assay Note: WST-based cell viability or mitochondrial function assays (e.g., CCK-8) can be used alongside SLU-PP-332 treatment

In Vivo
Model: C57BL/6J mice (normal and diet-induced obesity)

Dosage: 50 mg/kg via intraperitoneal injection

Regimen: Single or twice-daily for 12–28 days

Findings:

Elevated oxidative muscle markers (e.g., ATP5A, cytochrome c)

Increased fatty acid oxidation (FAO)

Decreased adipocyte size and fat mass in obese mice

No effect on food intake or brown adipose tissue mass

Solubility & Preparation
Solvent: DMSO

Solubility: 100 mg/mL (344.45 mM) with ultrasonication

Note: Use freshly opened, dry DMSO to ensure reproducibility

Avoid repeated freeze-thaw cycles by aliquoting stock solutions

Example Stock Solution (1 mM in DMSO):

Dissolve 1 mg in 3.44 mL DMSO

Store at –80°C for up to 6 months

Related Research Tools
Estrogen Receptor Antibodies: ERα, ERβ, ERRγ, GPR30

Mitochondrial Markers: PBR, Cytochrome C

Metabolic Regulators: PDK4, 14-3-3, MVK

SEO Keywords (for product visibility)
SLU-PP-332 ERR agonist

Estrogen-related receptor activator

Mitochondrial function enhancer

Skeletal muscle metabolism research

Pan-ERR modulator for metabolic disease

Small molecule ERRα/β/γ agonist